| Bioactivity | Rhodamine-N3 chloride is an azide-rhodamine fluorescent dye that can be used to label biomolecules containing alkyne groups[1][2]. |
| Invitro | The cell lysates or cells are treated with probes (0.001-10 μM, 1 hr), and then reacted with an Rhodamine-N3 (N3-Rh) reporter tag under copper-catalyzed azide-alkyne cycloaddition (CuACC or click chemistry) conditions and probe-labeled proteins visualized by SDS-PAGE and in-gel fluorescence scanning[2]. |
| Name | Rhodamine-N3 chloride |
| CAS | 2363751-90-8 |
| Formula | C44H59ClN8O7 |
| Molar Mass | 847.44 |
| Appearance | Solid |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | 4°C, sealed storage, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture) |
| Reference | [1]. Shijiao Cai, et al. Glycyrrhizic Acid-Induced Differentiation Repressed Stemness in Hepatocellular Carcinoma by Targeting c-Jun N-Terminal Kinase 1. Front Oncol. 2020 Jan 9;9:1431. [2]. Bryan R Lanning, et al. A road map to evaluate the proteome-wide selectivity of covalent kinase inhibitors. Nat Chem Biol. 2014 Sep;10(9):760-767. |