| Bioactivity | Quin-2AM is a fluorecent Ca2+ chelator, with high affinity for calcium. Quin-2AM can specifically identify intracellular calcium ions, with high sensitivity, low cytotoxicity, increased AM acetylmethyl ester can enter the cell well, after being sheared by the intracellular esterase stay in the cell to bind to calcium ions, produce strong fluorescence[1]. Storage: protect from light. |
| Invitro | 1. Quin-2AM 工作液的配制1.1 制备储存液用无水 DMSO 稀释 Quin-2AM 去配制 10 mM 的储备液。注：Quin-2AM 储存液建议分装后于 -20℃ 或 -80℃ 避光保存。1.2 工作液的配制用预热好的 HBSS 稀释储存液，配制成 1-10 μM 的 Quin-2AM 工作液。注：请根据实际情况调整 Quin-2AM 工作液浓度，且现用现配。2. 细胞染色 (悬浮细胞)2.1 离心收集细胞，加入 PBS 洗涤两次，每次 5 分钟。细胞密度在1×106/mL2.2 加入 1 mL Quin-2AM 工作液，室温孵育 5-30 分钟。2.3 400 g，离心 3-4 分钟，弃去上清。2.4 加入 PBS 洗涤细胞两次，每次 5 分钟。2.5 用 1 mL HBSS 重悬细胞后，使用荧光显微镜或流式细胞仪进行观察。3. 细胞染色 (贴壁细胞)3.1 将贴壁细胞培养于无菌盖玻片上。3.2 从培养基中移出盖玻片，吸除多余培养基。3.3 加入 100 μL 染料工作液，轻轻晃动使其完全覆盖细胞，孵育 5-30 分钟。3.4 吸去染料工作液，用培养基洗 2-3 次，每次 5 分钟，使用荧光显微镜进行观察。 |
| Name | Quin-2AM |
| CAS | 83104-85-2 |
| Formula | C38H43N3O18 |
| Molar Mass | 829.76 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Chu AJ, et al. Possible role of Marcks in the cellular modulation of monocytic tissue factor-initiated hypercoagulation. Br J Haematol. 2002 Aug;118(2):569-76. [2]. Chu AJ, et al. Possible role of Marcks in the cellular modulation of monocytic tissue factor-initiated hypercoagulation. Br J Haematol. 2002 Aug;118(2):569-76. |