| Bioactivity | Psalmotoxin 1 (PcTx1) TFA is a protein toxin that can bind at subunit-subunit interfaces of acid-sensing ion channel 1a (ASIC1a). Psalmotoxin 1 TFA is a potent and slective ASIC1a inhibitor (IC50: 0.9 nM) by increasing the apparent affinity for H+ of ASIC1a. Psalmotoxin 1 TFA can induce cell apoptosis, also inhibits cell migration, proferliration and invasion of cancer cells. Psalmotoxin 1 TFA can be used in the research of cancers, or neurological disease[1][3][4][6]. |
| Invitro | Psalmotoxin 1 (20 nM，125 s) TFA 通过显著改变稳态脱敏曲线以降低 H+ 浓度来抑制 ASIC1a 电流[1]。Psalmotoxin 1 (30 nM) TFA 与 Ca2+ 竞争结合 ASIC1a 通道[1]。Psalmotoxin 1 (100 或 200 ng，24-72 小时) TFA 显著减弱 MCF-7 和 MDA-MB-231 细胞的迁移、增殖和侵袭[4]。 Psalmotoxin 1 (100 ng/mL，24 h) 显著抑制酸诱导的增加参与细胞内钙和 LDH 释放，诱导髓核细胞 (NPC) 细胞凋亡和细胞周期停滞[5]。 MCE has not independently confirmed the accuracy of these methods. They are for reference only. 0 --> Psalmotoxin 1 TFA 相关抗体: Cell Proliferation Assay[4] Cell Line: |
| In Vivo | Psalmotoxin 1 (icv，1 ng/kg，单次剂量) TFA 通过直接抑制 ASIC1a 在有意识的中风模型中发挥神经保护作用[2]。Psalmotoxin 1 (尾静脉注射，10 ng/kg，每日一次，持续 7 天) TFA 抑制乳腺癌小鼠模型的肿瘤生长[4]。 MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: |
| Sequence | Glu-Asp-Cys-Ile-Pro-Lys-Trp-Lys-Gly-Cys-Val-Asn-Arg-His-Gly-Asp-Cys-Cys-Glu-Gly-Leu-Glu-Cys-Trp-Lys-Arg-Arg-Arg-Ser-Phe-Glu-Val-Cys-Val-Pro-Lys-Thr-Pro-Lys-Thr (Disulfide bridge: Cys3-Cys18, Cys10-Cys23, Cys17-Cys33) |
| Shortening | EDCIPKWKGCVNRHGDCCEGLECWKRRRSFEVCVPKTPKT (Disulfide bridge: Cys3-Cys18, Cys10-Cys23, Cys17-Cys33) |
| Formula | C200H312N62O57S6.xC2HF3O2 |
| Molar Mass | 4689.39 (free base) |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Chen X, et al. The tarantula toxin psalmotoxin 1 inhibits acid-sensing ion channel (ASIC) 1a by increasing its apparent H+ affinity. J Gen Physiol. 2005 Jul;126(1):71-9. [2]. Claudia A McCarthy, et al. PcTx1 affords neuroprotection in a conscious model of stroke in hypertensive rats via selective inhibition of ASIC1a. Neuropharmacology. 2015 Dec;99:650-7. [3]. Niko Joeres, et al. Functional and pharmacological characterization of two different ASIC1a/2a heteromers reveals their sensitivity to the spider toxin PcTx1. Sci Rep. 2016 Jun 9;6:27647. doi: 10.1038/srep27647. [4]. Chao Yang, et al. Overexpression of acid-sensing ion channel 1a (ASIC1a) promotes breast cancer cell proliferation, migration and invasion. Transl Cancer Res. 2020 Dec;9(12):7519-7530. [5]. Feng Cai, et al. Acid-sensing ion channel 1a regulates the survival of nucleus pulposus cells in the acidic environment of degenerated intervertebral discs. Iran J Basic Med Sci. 2016 Aug;19(8):812-820. [6]. P Escoubas, et al. Isolation of a tarantula toxin specific for a class of proton-gated Na+ channels. J Biol Chem. 2000 Aug 18;275(33):25116-21. |
Psalmotoxin 1 TFA
CAS: F: C200H312N62O57S6.xC2HF3O2 W: 4689.39 (free base)
Psalmotoxin 1 (PcTx1) TFA is a protein toxin that can bind at subunit-subunit interfaces of acid-sensing ion channel 1a
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