| Bioactivity | Ponceau S (Acid Red 112) is the most commonly used stain for Western blotting. Ponceau S is applied as an acidic aqueous solution. Ponceau S is compatible with antibody-antigen binding, and stains the proteins on the membrane red[1][2]. Storage: protect from light. | ||||||||||||
| Name | Ponceau S | ||||||||||||
| CAS | 6226-79-5 | ||||||||||||
| Formula | C22H12N4Na4O13S4 | ||||||||||||
| Molar Mass | 760.57 | ||||||||||||
| Appearance | Solid | ||||||||||||
| Transport | Room temperature in continental US; may vary elsewhere. | ||||||||||||
| Storage |
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| Reference | [1]. Sander H, et, al. Ponceau S waste: Ponceau S staining for total protein normalization. Anal Biochem. 2019 Jun 15; 575: 44-53. [2]. Litovchick. Staining the Blot for Total Protein with Ponceau S. Cold Spring Harb Protoc. 2020 Mar 2; 2020(3): 098459. |
Ponceau S
CAS: 6226-79-5 F: C22H12N4Na4O13S4 W: 760.57
Ponceau S (Acid Red 112) is the most commonly used stain for Western blotting. Ponceau S is applied as an acidic aqueous
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