| Bioactivity | Poly (I:C):Kanamycin (1:1) sodium is an isometric complex of Poly (I:C) (HY-135748) and Kanamycin (HY-16566). Poly(I:C) sodium, a synthetic analog of double-stranded RNA, is a TLR3 and retinoic acid-inducible gene I receptor (RIG-I and b>MDA5) agonist. Poly(I:C) sodium can be used as a vaccine adjuvant to enhance innate and adaptive immune responses and induce apoptosis in cancer cells[1][2]. Kanamycin is an orally active antibacterial agent (Gram-negative/positive bacteria) that inhibits translocation and causes miscoding by binding to the 70S ribosomal subunit. Kanamycin shows good inhibitory activity against Mycobacterium tuberculosis (susceptible and drug-resistant) and Klebsiella pneumoniae, and can be used in the research of tuberculosis and pneumonia[3][4][5][6]. |
| Target | TLR3, RIG-I, MDA5, apoptosis |
| Invitro | Poly (I:C) sodium（20 ng/mL；24 小时；WM793、WM278、WM239A、WM9 和 1205Lu 细胞）处理细胞后，可在 24 小时内显着降低存活率，从对照的 100% 降至 20%-50%[1].Poly (I:C) sodium（200 ng/mL；24 小时；1205Lu 细胞）处理诱导 1205Lu 细胞凋亡[1]。Polyinosinic-polycytidylic acid（3 ng/mL；24 小时；1205Lu 细胞）处理诱导黑色素瘤细胞中的 IFN-β 表达。 RIG-I 和 MDA-5 的沉默证实 Poly (I:C) sodium 诱导 IFN-β 分别需要 RIG-I 和 MDA-5，并且需要 IPS-1[1]。Poly (I:C) sodium（5 ng/mL；24 小时；1205Lu 细胞）处理揭示黑素瘤细胞中 caspase-9 和 caspase-8 的活性亚基[1]。Kanamycin (0.1-100 μg/mL; 2 周) 在体外对多种分枝杆菌菌株表现出良好的抗菌活性 (MIC=1-5 μg/mL)[3]。 |
| In Vivo | Poly (I:C) sodium 可抑制注射 1205Lu 细胞的 NOD/SCID 免疫缺陷小鼠的肿瘤生长。在用 Poly (I:C) sodium 处理的小鼠中，人类 DNA 的水平降低了 50%[1]。Kanamycin（2、4 mg/kg；皮下注射；每天一次，每周 6 次，持续 3 周）抑制小鼠肺和脾脏中牛结核杆菌的生长[3]。Kanamycin（1.25、5 mg/kg；皮下注射；感染后 3 小时，单次注射）抑制肺炎克雷伯菌 DT-S 在小鼠肺、气管和血液中的增殖，并且与给药剂量成比例，并且还会增加小鼠的存活率[4]。 |
| Name | Poly (I:C):Kanamycin (1:1) (sodium) |
| Appearance | Solid |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | -20°C, sealed storage, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture) |
| Reference | [1]. Robert Field, et al. Systemic challenge with the TLR3 agonist poly I:C induces amplified IFNalpha/beta and IL-1beta responses in the diseased brain and exacerbates chronic neurodegeneration. Brain Behav Immun. 2010 Aug;24(6):996-1007. [2]. Besch R, et al. Proapoptotic signaling induced by RIG-I and MDA-5 results in type I interferon-independent apoptosis in human melanoma cells. J Clin Invest. 2009 Aug;119(8):2399-411. [3]. Cheng YS, et al. Anticancer function of polyinosinic-polycytidylic acid. Cancer Biol Ther. 2010 Dec 15;10(12):1219-23. |