PeptideDB

Phalloidin

CAS: 17466-45-4 F: C35H48N8O11S W: 788.87

Phalloidin is a mushroom-derived toxin which can be used to label F-actin of the cytoskeleton with fluorochrome(λex=495
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Bioactivity Phalloidin is a mushroom-derived toxin which can be used to label F-actin of the cytoskeleton with fluorochrome(λex=495 nm, λem=520 nm).
Invitro Phalloidin staining will not work with methanol-fixed cells, probably because of the disruption of actin filament integrity, but it will work with cells fixed in 0.2% glutaraldehyde in PBS. For fluorescein isothiocyanate (FITC)-Phalloidin (or rhodamine isothiocyanate [RITC]-Phalloidin), the excitation wavelength maximum will be near 495 nm (or 550 nm) and the emission wavelength maximum will be ~520 nm based on H2O at pH 8.0 as the solvent (575 nm with methanol as the solvent). The emission spectrum of fluorescein can depend on the environmental pH and/or ionic strength[1].
Name Phalloidin
CAS 17466-45-4
Sequence Ala-{d-Thr}-Cys-{Hyp}-Ala-Trp-Leu (Sulfide bridge: Cys3-Trp6)
Shortening A-{d-Thr}-C-{Hyp}-AWL (Sulfide bridge: Cys3-Trp6)
Formula C35H48N8O11S
Molar Mass 788.87
Transport Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture and light

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)