| Bioactivity | Phalloidin is a mushroom-derived toxin which can be used to label F-actin of the cytoskeleton with fluorochrome(λex=495 nm, λem=520 nm). | ||||||
| Invitro | Phalloidin staining will not work with methanol-fixed cells, probably because of the disruption of actin filament integrity, but it will work with cells fixed in 0.2% glutaraldehyde in PBS. For fluorescein isothiocyanate (FITC)-Phalloidin (or rhodamine isothiocyanate [RITC]-Phalloidin), the excitation wavelength maximum will be near 495 nm (or 550 nm) and the emission wavelength maximum will be ~520 nm based on H2O at pH 8.0 as the solvent (575 nm with methanol as the solvent). The emission spectrum of fluorescein can depend on the environmental pH and/or ionic strength[1]. | ||||||
| Name | Phalloidin | ||||||
| CAS | 17466-45-4 | ||||||
| Sequence | Ala-{d-Thr}-Cys-{Hyp}-Ala-Trp-Leu (Sulfide bridge: Cys3-Trp6) | ||||||
| Shortening | A-{d-Thr}-C-{Hyp}-AWL (Sulfide bridge: Cys3-Trp6) | ||||||
| Formula | C35H48N8O11S | ||||||
| Molar Mass | 788.87 | ||||||
| Transport | Room temperature in continental US; may vary elsewhere. | ||||||
| Storage | Sealed storage, away from moisture and light
*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light) |