| Bioactivity | PKH67 is a fluorescent cell binding dye with green fluorescence. PKH67 can stain the cell membrane and the Ex/Em is 490/502 nm. PKH67 is often used in combination with the non-specific red fluorescent dye PKH26 (Ex/Em=551/567 nm) to label cells, detect cell proliferation in vitro, and trace cells in vitro and in vivo[1][2]. |
| Invitro | 用于胞葬试验的细胞标记方案（以 Jurkat 细胞为例）[1]：1. 诱导细胞凋亡：在 RPMI-1640 培养基中加入 5 μg/mL Staurosporine (HY-15141)，在 37°C，5% CO2 条件下，以 2.5×106 cells/mL 的密度孵育 3 h。2. 标记细胞：用 1× DPBS 中清洗 Jurkat 细胞，并在含有 PKH67 (绿色荧光) 或 PKH26 (红色荧光) 的 Diluent C 中以 2×107 细胞/mL 的浓度重悬细胞。3. 用含 10% HI-FBS 的 DMEM 基础培养基冲洗细胞两次。制备好的细胞应立即用于胞吐试验。 |
| Name | PKH67 |
| CAS | 257277-27-3 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Shi J, et al. A genome-wide CRISPR screen identifies WDFY3 as a regulator of macrophage efferocytosis. Nat Commun. 2022 Dec 24;13(1):7929. [2]. He L, et al. Intelligent manganese dioxide nanocomposites induce tumor immunogenic cell death and remould tumor microenvironment[J]. Chemical Engineering Journal, 2023: 141369. |