| Bioactivity | PFB-FDG is a non-fluorescent galactosidase substrate that can be hydrolysed to green fluorescent PFB-F (Ex=485 nm, Em=535 nm). PFB-FDG can be used for the determination of β-galactosidase activity[1]. |
| Invitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Measurement of β-galactosidase activity[1]:1. Incubate 2 × 105 cells with 50 µg/mL of PFB-FDG in serum-free DMEM containing 25 mM Hepes (pH 7.4) for 2 h at 37℃. (Mononuclear cells for example, different cell concentrations vary)2. Wash cells once with ice-cold PBS.3. Analyze cells by flow cytometry (Ex=485 nm, Em=535 nm). |
| Name | PFB-FDG |
| CAS | 209540-64-7 |
| Formula | C39H32F5NO16 |
| Molar Mass | 865.66 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Navarrete Santos A, et al. Evidences for age-related modulation of human hematopoietic progenitor cell proliferation. Exp Gerontol. 2008 Nov;43(11):1033-8. |
PFB-FDG
CAS: 209540-64-7 F: C39H32F5NO16 W: 865.66
PFB-FDG is a non-fluorescent galactosidase substrate that can be hydrolysed to green fluorescent PFB-F (Ex=485 nm, Em=53
Sales Email：peptidedb@qq.com
This product is for research use only, not for human use. We do not sell to patients.