| Bioactivity | PETCM is an activator of caspase-3 and acts as an cytochrome c (cyto c)-dependent manner. PETCM promotes Apaf-1 oligomerization and induces cell apoptosis in HeLa cells[1][2]. | ||||||||||||
| Invitro | PETCM (0.1-0.5 mM) stimulates caspase-3 activity (DEVD activity) of HeLa S-100 in a dose-dependent manner.And 0.2 mM PETCM is more efficient in activating caspase-3 than 1 mM dATP[1].PETCM (0.2 mM; 1 hour) drives apoptosome formation. Apaf-1 in a normal HeLa cell S-100 fraction is in an inactive monomeric form. After 1 mM dATP, most of the Apaf-1 shifted to a size of 1 million daltons. After the S-100 fraction with 0.2 mM PETCM, Apaf-1 exhibits a similar shift. And the efficiency of apoptosome formation was better with 0.2 mM PETCM[1].PETCM (0.2 mM; 1 hour) can antagonize the inhibitory activity of ProT reduced caspase-3 activation in in HeLa cells[1]. Western Blot Analysis[1] Cell Line: | ||||||||||||
| Name | PETCM | ||||||||||||
| CAS | 10129-56-3 | ||||||||||||
| Formula | C8H8Cl3NO | ||||||||||||
| Molar Mass | 240.51 | ||||||||||||
| Transport | Room temperature in continental US; may vary elsewhere. | ||||||||||||
| Storage |
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