| Bioactivity | ODN 1668, a class B CpG ODN (oligodeoxynucleotide), is a TLR-9 agonist. ODN 1668 is an immunostimulatory sequence and can be used as vaccine adjuvant. Sequence: 5'-tccatgacgttcctgatgct-3’[1][2]. |
| Invitro | ODN 1668 induces TNF-α secretion and promotes polyclonal B cell activation[1]. |
| In Vivo | ODN 1668 (10 nmol) stimulates responses to protein antigen[1].ODN 1668 (1 or 5 mg/kg; i.p. or s.c.; once) causes moderate fever and anorexia in rats[2]. Animal Model: |
| Name | ODN 1668 |
| CAS | 1186063-66-0 |
| Sequence | DNA, d(P-thio)(T-C-C-A-T-G-A-C-G-T-T-C-C-T-G-A-T-G-C-T) |
| Molar Mass | 6364.10 |
| Appearance | Solid |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | -20°C, sealed storage, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture) |
| Reference | [1]. Lipford GB, et al. CpG-containing synthetic oligonucleotides promote B and cytotoxic T cell responses to protein antigen: a new class of vaccine adjuvants. Eur J Immunol. 1997 Sep;27(9):2340-4. [2]. Damm J, et al. Intraperitoneal and subcutaneous injections of the TLR9 agonist ODN 1668 in rats: brain inflammatory responses are related to peripheral IL-6 rather than interferons. J Neuroimmunol. 2014 Dec 15;277(1-2):105-17. |