| Bioactivity | Neutral Red, a nitrogenous pH-indicator with a pKi of 6.8, is an indicator for the internal acidification of thylakoids. Neutral Red stains lysosomes red[1]. |
| Invitro | As cells begin to die, their ability to incorporate neutral red diminishes. The neutral red is also used to stain cell cultures for plate titration of viruses[2]. |
| Name | Neutral Red |
| CAS | 553-24-2 |
| Formula | C15H17ClN4 |
| Molar Mass | 288.78 |
| Appearance | Solid |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | 4°C, sealed storage, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture) |
| Reference | [1]. Winfried AUSLANDER, et al. NEUTRAL RED, A RAPID INDICATOR FOR pH-CHANGES IN THE INNER PHASE OF THYLAKOIDS. FEBS LETTERS. 1975 Nov. [2]. Ellen Borenfreund, et al. A simple quantitative procedure using monolayer cultures for cytotoxicity assays (HTD/NR-90). Journal of tissue culture methods volume 9, pages7–9 (1985). |