| Bioactivity | Ned-K is a nicotinic acid adenine dinucleotide phosphate (NAADP) antagonist. Ned-K is effective at dampening simulated ischaemia and reperfusion (sIR)-induced Ca2+ oscillations in cardiomyocytes[1]. |
| Invitro | Ned-K suppresses Ca2+ oscillations and dramatically protects cardiomyocytes from cell death in vitro after ischaemia and reoxygenation, preventing opening of the mitochondrial permeability transition pore. Ned-K (10 µM) almost completely eliminates [Ca2+]c oscillations, and Ned-K (0.1 µM) is effective at suppressing [Ca2+]c levels[1]. Cell Viability Assay[1] Cell Line: |
| In Vivo | Injection of Ned-K causes a significant reduction in infarct size in mice. Ned-K (administered i.v. to mice 5 min before reperfusion) significantly decreases myocardial infarct size relative to area at risk[1]. |
| Name | Ned-K |
| CAS | 2250019-90-8 |
| Formula | C31H31N5O3 |
| Molar Mass | 521.61 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Sean M Davidson, et al. Inhibition of NAADP signalling on reperfusion protects the heart by preventing lethal calcium oscillations via two-pore channel 1 and opening of the mitochondrial permeability transition pore. Cardiovasc Res. 2015 Dec 1;108(3):357- |