| Bioactivity | NBD-Cl is a nonfluorescent reagent which becomes highly fluorescent after reaction with thiol or amino groups[1]. Storage: protect from light. |
| Invitro | NBD-Cl (NBD chloride) forms highly fluorescent derivatives for detection of all the protein amino acids. In addition, NBD-Cl provides a simple and sensitive method for determination of N-terminal amino acids. The differences in intensity and color of fluorescence can be used to advantage to identify prolyl peptides[1]. |
| Name | NBD-Cl |
| CAS | 10199-89-0 |
| Formula | C6H2ClN3O3 |
| Molar Mass | 199.55 |
| Appearance | Solid |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | 4°C, protect from light *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light) |
| Reference | [1]. Fager RS, et al. The use of NBD chloride (7 chloro-4-nitrobenzo-2-oxa-1,3-diazole) in detecting amino acids and as an N-terminal reagent. Anal Biochem. 1973 May;53(1):290-4. |