Merocyanin 540_product_DataBase

Bioactivity	Merocyanin 540 is a fluorescent membrane probe that selectively stains the membranes of a wide variety of electrically excitable cells, but not those of nonexcitable cells (Ex/Em: 540/580 nm)[1][2].
Invitro	Guidelines[1] (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Labeling of Cells: 1. Wash monolayer cultured cells with isotonic saline with or without 1-5 mM CaCl2 or iso-osmotic sucrose (0.25 M) solutions buffered with 5 mM Tris-Cl (pH 7.4). 2. Expose cells to Merocyanin 540 (1.6 x 10-5 M) in the appropriate buffer for 10 min at room temperature under ambient lighting conditions. 3. Wash extensively and examine by fluorescence microscopy. 4. For protozoa, place 0.05 mL aliquots of washed cultures containing 100-200 organisms on a microscope slide. 5. Add Merocyanin 540 to a final concentration of 1.0-10 μg/mL. 6. Examine cells by fluorescence microscopy. 7. Incubate single cell suspensions or tissue slices with Merocyanin 540 (1.75-5.25 x 10-5 M) and examine by fluorescence microscopy as described for attached cells.
Name	Merocyanin 540
CAS	62796-23-0
Formula	C26H32N3NaO6S2
Molar Mass	569.67
Transport	Room temperature in continental US; may vary elsewhere.
Storage	Please store the product under the recommended conditions in the Certificate of Analysis.
Reference	[1]. Easton TG, et al. Merocyanine 540 as a fluorescent probe of membranes: staining of electrically excitable cells. Cell. 1978 Mar;13(3):475-86. [2]. Lagerberg JW, et al. Factors affecting the amount and the mode of merocyanine 540 binding to the membrane of human erythrocytes. A comparison with the binding to leukemia cells. Biochim Biophys Acta. 1995 May 4;1235(2):428-36.

PeptideDB

Merocyanin 540

CAS: 62796-23-0 F: C26H32N3NaO6S2 W: 569.67