| Bioactivity | Malantide TFA is a synthetic dodecapeptide derived from the site phosphorylated by cAMP-dependent protein kinase (PKA) on the β-subunit of phosphorylase kinase. Malantide TFA is a highly specific substrate for PKA with a Km of 15 μM and shows protein inhibitor (PKI) inhibition >90% substrate phosphorylation in various rat tissue extracts[1]. Malantide TFA is also an efficient substrate for PKC with a Km of 16 μM[2]. |
| Target | Km: 15 μM (PKA), 16 μM (PKC), 233 μM (PKG) |
| Invitro | The Km values of Malantide are 15 μM and 223 μM for PKA and PKG, respectively. The Vmax values are 23.8 units/mg and 6.6 units/mg for for PKA and PKG, respectively[1].A statistically significant effect of isoprenaline on the activity ratio of guinea-pig heart was only: observed with Malantide[1]. |
| Name | Malantide TFA |
| Sequence | Arg-Thr-Lys-Arg-Ser-Gly-Ser-Val-Tyr-Glu-Pro-Leu-Lys-Ile |
| Shortening | RTKRSGSVYEPLKI |
| Formula | C74H125F3N22O23 |
| Molar Mass | 1747.91 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. K J Murray, et al. Use of a Synthetic Dodecapeptide (Malantide) to Measure the Cyclic AMP-dependent Protein Kinase Activity Ratio in a Variety of Tissues. Biochem J. 1990 May 1;267(3):703-8. [2]. Z H Zhao, et al. Characterization of a New Substrate for Protein Kinase C: Assay by Continuous Fluorometric Monitoring and High Performance Liquid Chromatography. Biochem Biophys Res Commun. 1991 May 15;176(3):1454-61. |