LysoTracker Yellow HCK 123_product_DataBase

Bioactivity	LysoTracker Yellow HCK 123 is a potent yellow membrane-permeable fluorescent probe. LysoTracker Yellow HCK 123 is a weakly basic amine that selectively accumulates in cellular compartments with low luminal pH. (λex=465 nm, λem=535 nm)[1].
Invitro	Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).LysoTracker Yellow HCK 123 assay[1] (to quantify GAPDH):1. Culture EMT6 cells in 8-well cell culture slides. Incubate the cells according to your normal protocol.2. Add TONS504 per well to make the final concentration at 30 µg/mL.Incubate cells at 37 °C for 4 hours.3. Add LysoTracker Yellow HCK 123 per well to make the final concentration at 50 μM. Incubate cells at 37 °C, 5% CO2, for 30 minutes.4. Analyze samples on a confocal laser scanning microscope.
Name	LysoTracker Yellow HCK 123
CAS	1064123-31-4
Formula	C16H24N6O4
Molar Mass	364.40
Transport	Room temperature in continental US; may vary elsewhere.
Storage	Please store the product under the recommended conditions in the Certificate of Analysis.
Reference	[1]. Desclés J, et, al. New tools for labeling silica in living diatoms. New Phytol. 2008;177(3):822-829.

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