| Bioactivity | ITX3 is a specific and nontoxic inhibitor of TrioN (N-terminal GEF domain of the multidomain Trio protein) with an IC50 value of 76 μM. ITX3 can be used for the research of drug[1][2]. | ||||||||||||
| Target | IC50: 76 μM (TrioN) | ||||||||||||
| Invitro | ITX3 (5, 10, 25, 50 and 100 μM; 24 h) inhibits TrioN signaling[1].ITX3 (50 μM; 1 h) specifically inhibits TrioN[1].ITX3 (100 μM; 36 h) inhibits nerve growth factor-induced neurite outgrowth in PC12 cells[1].ITX3 (1, 10 and 100 μM) represses Rac1 activity and dose-dependently up-regulates the E-cadherin protein level and phospho-p38 signal in Tara-KD cells[2]. Western Blot Analysis[1] Cell Line: | ||||||||||||
| Name | ITX3 | ||||||||||||
| CAS | 347323-96-0 | ||||||||||||
| Formula | C22H17N3OS | ||||||||||||
| Molar Mass | 371.45 | ||||||||||||
| Appearance | Solid | ||||||||||||
| Transport | Room temperature in continental US; may vary elsewhere. | ||||||||||||
| Storage |
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| Reference | [1]. Bouquier N, et al. A cell active chemical GEF inhibitor selectively targets the Trio/RhoG/Rac1 signaling pathway. Chem Biol. 2009 Jun 26;16(6):657-66. [2]. Yano T, et al. Tara up-regulates E-cadherin transcription by binding to the Trio RhoGEF and inhibiting Rac signaling. J Cell Biol. 2011 Apr 18;193(2):319-32. |