Fluorescein diphosphate tetraammonium_product_DataBase

Bioactivity	Fluorescein diphosphate tetraammonium is a fluorescent dye, it can be used as a fluorogenic substrate and generats fluorescein as a fluorescent product[1][2][3].
Invitro	Fluorescein diphosphate tetraammonium can be used as a fluorogenic substrate, and obtains luorescein as a fluorescent product[1].Fluorescein diphosphate tetraammonium (50 μM; room temperature) can be used as a substrate in vitro phosphatase assays[2].Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).ELISA with capillary immunosensor (single-step ELISA)[3]:1. Put various concentrations of antigen in Tris buffer 1 containing 1% BSA into capillary immunosensor.2. Immobilize capture antibody and enzyme-linked antibody via capillary action, incubates for a preset time, and subsequently washing with 1 mL of 50 mM Tris buffer (pH 7.4) containing 0.14 M NaCl and 0.05% Tween 20 by using a syringe.3. Fluorescence is detected by introducing an excess volume (ca. 100 μL) of 20 μM FDP in 50 mM Tris buffer (pH 9) with an incubation time of 60 min.
Name	Fluorescein diphosphate tetraammonium
CAS	217305-49-2
Formula	C20H26N4O11P2
Molar Mass	560.39
Transport	Room temperature in continental US; may vary elsewhere.
Storage	Please store the product under the recommended conditions in the Certificate of Analysis.
Reference	[1]. Murakami Y, et al. On-chip capillary electrophoresis for alkaline phosphatase testing. Biosens Bioelectron. 2001 Dec;16(9-12):1009-14. [2]. Gilmartin AG, et al. Allosteric Wip1 phosphatase inhibition through flap-subdomain interaction. Nat Chem Biol. 2014 Mar;10(3):181-7. [3]. Tsutsumi E, et al. Single-step sandwich immunoreaction in a square glass capillary immobilizing capture and enzyme-linked antibodies for simplified enzyme-linked immunosorbent assay. Anal Sci. 2012;28(1):51-6.

PeptideDB

Fluorescein diphosphate tetraammonium

CAS: 217305-49-2 F: C20H26N4O11P2 W: 560.39