| Bioactivity | FDGlcU can be used as a fluorescent probe for non-invasively image with a high level of fluorescent activity. FDGlcU is non-fluorescent when the fluorescein is conjugated with two mono-glucuronides (Ex/Em=480/514 nm)[1]. |
| Invitro | FDGlcU (0.5 μg/mL; 12 h) shows enzymatic activity and inhibits βG activity in live bacteria[1]. |
| In Vivo | FDGlcU (7.3 μM/kg) can be used for in vivo time-lapse imaging of bacterial βG activity[1]. FDGlcU can be used for real-time imaging in vivo[1].Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Whole-body imaging for intestinal βG activity[1]:1. On the day of imaging, gavage mice with 50 μL of DDW, FDGlcU (7.3 μM/kg), and fluorescein (7.3 μM/kg). 2. Anesthetize mice with isoflurane and whole-body optical images using an IVIS spectrum optical imaging system with a GFP filter set. 3. In biodistribution study, mice are sacrificed 3 hour after gavaged with FDGlcU or fluorescein (λex =480 nm, λem =514 nm). |
| Name | FDGlcU |
| CAS | 209540-67-0 |
| Formula | C39H28F5NO18 |
| Molar Mass | 893.63 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Chen M, et al. Real-time imaging of intestinal bacterial β-glucuronidase activity by hydrolysis of a fluorescent probe. Sci Rep. 2017 Jun 9;7(1):3142. |