FD-1080_product_DataBase

Bioactivity	FD-1080 is a fluorophore with both excitation and emission in the NIR-II region (Ex=1064 nm, Em=1080 nm). FD-1080 can be used for in vivo imaging[1].
Invitro	1. FD-1080 工作液的配制1.1 制备储存液用 DMSO 稀释 FD-1080 配制 10 mM 的 FD-1080。注：FD-1080 储存液建议分装后于 -20 ℃ 或 -80 ℃ 避光保存。1.2 工作液的配制用预热好的PBS 稀释储存液，配制成 2-10 μM 的 FD-1080 工作液。0.2 μm 滤膜过滤除菌。注：请根据实际情况调整 FD-1080 工作液浓度，且现用现配。2. 细胞实验2.1 悬浮细胞：离心收集细胞，加入 PBS 洗涤两次，每次 5 分钟。贴壁细胞：弃去培养基，加入胰蛋白酶消化细胞。离心弃去上清后，加入 PBS 洗涤两次，每次 5 分钟。2.2 加入 1 mL FD-1080 工作液，室温孵育 10-30 分钟。2.3 400 g，4℃ 离心 3-4 分钟，弃去上清。2.4 加入 PBS 洗涤细胞两次，每次 5 分钟。2.5 用 1 mL 无血清培养基或 PBS 重悬细胞后，在荧光显微镜或流式细胞仪下检测。3. 活体注射3.1 将 200 μL 80 μM FD-1080工作液静脉注射至小鼠体内，10-20 分钟后进行体内成像分析。
In Vivo	The 1064 nm NIR-II excitation of FD-1080 is demonstrated with the high tissue penetration depth and superior imaging resolution compared to NIR excitation from 650 nm to 980 nm. Deeptissue and high-resolution in vivo imaging for the left hindlimb vasculature, abdomen, and brain vessels was realized, allowing penetration through intact skin, tissue, and skull. FD-1080 also quantifying the respiratory rate based on the dynamic imaging of respiratory craniocaudal motion of the liver for the awake and anaesthetized mouse[1].
Name	FD-1080
CAS	1151666-58-8
Formula	C40H38ClN2NaO6S2
Molar Mass	765.31
Appearance	Solid
Transport	Room temperature in continental US; may vary elsewhere.
Storage	4°C, sealed storage, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Reference	[1]. Li B, et al. An Efficient 1064 nm NIR-II Excitation Fluorescent Molecular Dye for Deep-Tissue High-Resolution Dynamic Bioimaging. Angew Chem Int Ed Engl. 2018 Jun 18;57(25):7483-7487. [2]. Benhao Li, et al. An Efficient 1064 nm NIR-II Excitation Fluorescent Molecular Dye for Deep-Tissue High-Resolution Dynamic Bioimaging. Angew Chem Int Ed Engl. 2018 Jun 18;57(25):7483-7487.

PeptideDB

FD-1080

CAS: 1151666-58-8 F: C40H38ClN2NaO6S2 W: 765.31