| Bioactivity | FBBBE is used to detect the production of H2O2 by cells. FBBBE can be triggered by intracellular H2O2 and converted to fluorescein, resulting in an increase in intracellular fluorescence (Ex=4480 nm, Em=512 nm)[1]. |
| Target | H2O2 |
| Invitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1].1. The RAW 264.7 cells are seeded at 250,000 in glass bottom 35 mm dishes and post-adhering (5 h). 2. Some dishes are treated with 1 μg/mL of LPS for 24 h. Other dishes are treated the next day after plating with either 100 μM H2O2 for 1 h or PBS for 1 h. 3. Cells are washed twice with PBS and fixed in ice-cold 95% ethanol for 15 min. 3. Cells are twice rinsed with PBS and incubated with either 50 μM probe (FBBBE) for 1 h at room temperature. 4. Cells are washed twice with PBS and coverslips were mounted over cells using Aqua Mount. 5. Confocal images are obtained at 80× magnification on an Olympus FV1000 confocal laser scanning microscope. 6. Images were processed using ImageJ software. |
| Name | FBBBE |
| CAS | 1522117-83-4 |
| Formula | C46H46B2O9 |
| Molar Mass | 764.47 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Daniel, K.B., et al. Readily accessible fluorescent probes for sensitive biological imaging of hydrogen peroxide. Chembiochem 14(5), 593-598 (2013). |