| Bioactivity | E235 is an expression regulator of activates transcription factor 4 (ATF4). E235 reduces cell viability by activating integrated stress response (ISR) and DNA damage response signals. E235 has anti-proliferative activity and can be used for tumor research[1]. |
| Target | Activates transcription factor 4, ATF4 |
| Invitro | E235 (1 µM；2、4、8、16 或 24 h) 以时间依赖性方式下调了 HT1080 细胞中 XBP-1s mRNA 的水平[1]。E235 (0.1-10 µM；4 d 或 5 d) 对 HT1080、RPMI-8226、B16F10、4T1、HT1080 shNT 和 HT1080 shATF4 细胞具有抗增殖活性[1]。E235 (0、1、5 和 10 µM；4 h) 以剂量依赖性方式增加了 AG1522 细胞中 p53 的表达。E235 (1 µM；2 h) 增加了 AG1522 细胞中 p-Chk2 的表达[1]。E235 (0、1、5 和 10 µM；2 h) 增加了 AG1522 和 HT1080 细胞中 p-p53 的表达[1]。E235 (0、0.5、1、5 和 10 µM；0.5 h) 以剂量依赖性方式增加了 HT1080 细胞中 γ-H2AX 的表达[1]。 Western Blot Analysis[1] Cell Line: |
| Name | E235 |
| CAS | 891894-69-2 |
| Formula | C28H25FN4OS |
| Molar Mass | 484.59 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Sayers CM, et al. Identification and characterization of a potent activator of p53-independent cellular senescence via a small-molecule screen for modifiers of the integrated stress response. Mol Pharmacol. 2013 Mar;83(3):594-604. |