| Bioactivity | Dermaseptin TFA, a peptide isolated from frog skin, exhibits potent antimicrobial activity against bacteria, fungi, and protozoa at micromolar concentration[1]. | ||||||
| Invitro | Dermaseptin TFA is a water-soluble, thermostable, and nonhemolytic peptide endowed with highly potent antimicrobial activity against pathogenic fungi at micromolar concentration. Circular dichroism spectra of Dermaseptin TFA in hydrophobic media indicated 80% alpha-helical conformation, and predictions of secondary structure suggested that Dermaseptin TFA can be configured as an amphiphatic alpha-helix spanning over residues 1-27, a structure that perturbs membrane functions regulating water flux[1]. Dermaseptin TFA exerts a lytic action upon bacteria, protozoa, yeasts, and filamentous fungi at micromolar concentrations. Molecular elements responsible for the exceptional antimicrobial potency of Dermaseptin TFA are to be traced to the NH2-terminal alpha-helical amphipathic segment spanning residues 1-18 of the molecule[1].Dermaseptin TFA (5-100 μg/ml; 48 hours) inhibits by 100% the proliferation of most microorganisms tested, including Gram-positive or Gram-negative bacteria, parasites, yeasts, and filamentous fungi, at micromolar concentrations[2]. Dermaseptin TFA (5-100 μg/ml; 48 hours) does not inhibit the proliferation of human KJ3 cells after a 48 h incubation, and Dermaseptin TFA treatment for 1 h does not permeate guinea pig lymphocytes up to the highest concentration assayed (200 μg/ml). Hemolysis of rabbit erythrocytes occurrs after 1 h of treatment at doses above 200 μg/ml, with 50% hemolysis at 350 μg/ml[2]. Dermaseptin TFA has antimicrobial activities and is against Aeromonas cauiae, Pseudomonas aeroginusa, Escherichia coli, Enterococcus faecalis, L. mezicana (NFα strain) and Microsporum canis (IP1194) with MIC values of 50 μg/ml; 100 μg/ml; 25 μg/ml; 15 μg/ml; and 50 μg/ml, respectively[2]. | ||||||
| Name | Dermaseptin TFA | ||||||
| Sequence | Ala-Leu-Trp-Lys-Thr-Met-Leu-Lys-Lys-Leu-Gly-Thr-Met-Ala-Leu-His-Ala-Gly-Lys-Ala-Ala-Leu-Gly-Ala-Ala-Ala-Asp-Thr-Ile-Ser-Gln-Gly-Thr-Gln | ||||||
| Shortening | ALWKTMLKKLGTMALHAGKAALGAAADTISQGTQ | ||||||
| Formula | C154H258N43F3O46S2 | ||||||
| Molar Mass | 3569.12 | ||||||
| Transport | Room temperature in continental US; may vary elsewhere. | ||||||
| Storage | Sealed storage, away from moisture and light
*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light) |
Dermaseptin TFA
CAS: F: C154H258N43F3O46S2 W: 3569.12
Dermaseptin TFA, a peptide isolated from frog skin, exhibits potent antimicrobial activity against bacteria, fungi, an
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