| Bioactivity | DPNB-ABT594 is a nitrobenzyl-caged ABT594 (HY-14316A) and activates nAChRs containing the α4β2 subunits with good selectivity than the α7 subunit. DPNB-ABT594 can be used to map the distribution of nAChRs on neurons of the medial habenula (MHb) and helps to gain a deeper understanding of the nAChR‐mediated Ca2+ signalling in the MHb[1]. |
| Target | Target: α4β2 nAChR; α7 nAChR |
| Invitro | DPNB‐ABT594 (20 μM) exhibits large inward currents and Ca2+ transients in response to laser uncaging at 410 nm at the soma, which increases linearly with energy dosage as expected for one‐photon excitation (1 mW, 0.5-3 ms)[1]. DPNB‐ABT594 is suitable for one- or two-photon photolysis, it evokes large inward currents and Ca2+ transients on cell bodies and dendrites of medial habenular neurons in mouse brain slices, following exposure to 410 nm light[1]. |
| Name | DPNB-ABT594 |
| Formula | C31H46ClN3O11 |
| Molar Mass | 672.16 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Stefan Passlick, et al. Optical Probing of Acetylcholine Receptors on Neurons in the Medial Habenula With a Novel Caged Nicotine Drug Analogue.J Physiol. 2018 Nov;596(22):5307-5318. |