| Bioactivity | DI-82 is a potent deoxycytidine kinase (dCK) inhibitor with an IC50app of 27.8 nM and Kiapp of 9.2 nM. DI-82 has antitumor activity[1]. | ||||||||||||
| Target | IC50app: 27.8 nM (dCK)Kiapp: 9.2 nM (dCK) | ||||||||||||
| Invitro | DI-82 (compound 12R) has an IC50 of 3.7 nM in CCRF-CEM acute lymphoblastic leukemia cells[1]. DI-82 (1 μM) has a NADPH-dependent CLint of 22.7 μL/min•mg and a NADPH-dependent T1/2 of 102 mins in a standard microsomal liver clearance assay[1]. DI-82 (200 μM) completely blunts the ability of decitabine to bind human thymidylate synthase (TS)[2]. | ||||||||||||
| Name | DI-82 | ||||||||||||
| CAS | 1638148-50-1 | ||||||||||||
| Formula | C20H26N6O4S3 | ||||||||||||
| Molar Mass | 510.65 | ||||||||||||
| Appearance | Solid | ||||||||||||
| Transport | Room temperature in continental US; may vary elsewhere. | ||||||||||||
| Storage |
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| Reference | [1]. Julian Nomme, et al. Structure-guided Development of Deoxycytidine Kinase Inhibitors With Nanomolar Affinity and Improved Metabolic Stability. J Med Chem. 2014 Nov 26;57(22):9480-94. [2]. Helena Almqvist, et al. CETSA Screening Identifies Known and Novel Thymidylate Synthase Inhibitors and Slow Intracellular Activation of 5-fluorouracil. Nat Commun. 2016 Mar 24;7:11040. |