| Bioactivity | Cypate, a cyanine dye, is a near infrared (NIR) fluorescent probe for in vivo tumor imaging[1][2]. |
| Invitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).1. 将每种类型细胞在单独的 35 毫米玻璃底培养皿上接种 1×106 个。2. 24 小时后，将细胞与 10 µM Cypate 无酚红培养基溶液一起孵育 2 小时。3. 处理后，细胞用 PBS 洗涤 3 次并用 4% 多聚甲醛固定用于成像，或收集细胞内的 Cypate 内容物用于光谱和/或 LC-MS 分析。4. 对体外细胞培养实验进行共聚焦显微镜检查。Cypate 的激发波长为 647 nm[1]。 |
| In Vivo | Cypate (10 nmol; 100 µL; 静脉给药; 每 24 小时一次; 连续 6 天) 导致肝脏蓄积最高[1]。Cypate (5 mg/kg; 静脉给药) 在具有 4T1 细胞的 Balb/c 小鼠肿瘤中的荧光信号微弱并迅速衰减，这可能是由于游离 Cypate 从体内快速消除所致。在游离cypate组中观察到肝脏和肾脏中更强的荧光。使用 IVIS Lumina 成像系统 (Ex-745 nm; Em-800 nm) 评估 Cypate 荧光信号[2]。 Animal Model: |
| Name | Cypate |
| CAS | 95837-47-1 |
| Formula | C41H40N2O4 |
| Molar Mass | 624.77 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Mona Doshi, et al. Cypate and Cypate-Glucosamine as Near-Infrared Fluorescent Probes for In Vivo Tumor Imaging. Mol Pharmacol. 2019 May;95(5):475-489. [2]. Yuanyuan Li, et al. Light-Decomposable Polymeric Micelles with Hypoxia-Enhanced Phototherapeutic Efficacy for Combating Metastatic Breast Cancer. Pharmaceutics. 2022 Jan 21;14(2):253. |