Clenbuterol_product_DataBase

Bioactivity	Clenbuterol (NAB-365) is a β2-adrenergic receptor agonist with an EC50 of 31.9 nM[1]. Clenbuterol is a very potent inhibitor of the lipopolysaccharide (LPS)-induced release of TNF-α and IL-1β. Clenbuterol can inhibit the inflammatory process. Clenbuterol is a bronchodilator[2].
Invitro	Clenbuterol (NAB-365) is a selective β2-adrenergic agonist (β2/β1 ratio = 4.0). Clenbuterol is a very potent inhibitor of the lipopolysaccharide (LPS)-induced release of TNF-α and IL-1β. Clenbuterol can inhibit the inflammatory process[2].Clenbuterol (10-200 µM; for 24 or 48 hours) decreases the viability of C2C12 myoblasts. Clenbuterol (100 µM) significantly decreases DNA synthesis. Clenbuterol (100 µM; for 12 h) increases the proportion of cells in G0/G1 phase. Clenbuterol treatment delays cell cycle progression. Clenbuterol (100 µM) induces cell cycle arrest, but not apoptosis, in C2C12 myoblasts[3]. Cell Viability Assay[3] Cell Line:
In Vivo	Treatment with Clenbuterol increases survival, rescues abnormalities in respiratory function and social recognition, and improves motor coordination in young male Mecp2-null (Mecp2−/y) mice[4]. Clenbuterol is a bronchodilator. Clenbuterol (90 µg) is administered intratracheally to five horses. Peak serum concentrations of ∼230 pg/mL are detected 10 min after administration, dropping to ∼50 pg/mL within 30 min and declining much more slowly thereafter. Intratracheal administration of clenbuterol shortly before race time can be detected with this serum test[5]. Animal Model:
Name	Clenbuterol
CAS	37148-27-9
Formula	C12H18Cl2N2O
Molar Mass	277.19
Transport	Room temperature in continental US; may vary elsewhere.
Storage	Please store the product under the recommended conditions in the Certificate of Analysis.
Reference	[1]. Gilad Barnea, et al. The genetic design of signaling cascades to record receptor activation. Proc Natl Acad Sci U S A. 2008 Jan 8;105(1):64-9. [2]. Olga Witkowska-Piłaszewicz, et al. The Effect of the Clenbuterol-β2-Adrenergic Receptor Agonist on the Peripheral Blood Mononuclear Cells Proliferation, Phenotype, Functions, and Reactive Oxygen Species Production in Race Horses In Vitro. Cells. 2021 Apr [3]. Min Chen, et al. Clenbuterol Induces Cell Cycle Arrest in C2C12 Myoblasts by Delaying p27 Degradation through β-arrestin 2 Signaling. Int J Biol Sci. 2017 Oct 17;13(10):1341-1350. [4]. Nikolaos Mellios, et al. β2-Adrenergic receptor agonist ameliorates phenotypes and corrects microRNA-mediated IGF1 deficits in a mouse model of Rett syndrome. Proc Natl Acad Sci U S A. 2014 Jul 8;111(27):9947-52. [5]. A F Lehner, et al. Clenbuterol in the horse: confirmation and quantitation of serum clenbuterol by LC-MS-MS after oral and intratracheal administration. J Anal Toxicol. May-Jun 2001;25(4):280-7.

PeptideDB

Clenbuterol

CAS: 37148-27-9 F: C12H18Cl2N2O W: 277.19