| Bioactivity | CHAPS, a derivative of Cholic acid, is a zwitterionic detergent for solubilizing membrane proteins. CHAPS is used for stabilization of various protein-DNA complexes and can retain biochemical activity of proteins in solution[1][2]. | ||||||||||||
| Invitro | CHAPS (0.5 %) is able to stabilize complexes between DNA and DNA-binding factors such as AP-1, SPI, GATA-1 and α-regulated factor ISGF3, and retains their biochemical activity[2].CHAPS can prevent dissociation of mononucleosomes diluted to sub-nanomolar concentrations[2]. | ||||||||||||
| Name | CHAPS | ||||||||||||
| CAS | 75621-03-3 | ||||||||||||
| Formula | C32H58N2O7S | ||||||||||||
| Molar Mass | 614.88 | ||||||||||||
| Appearance | Solid | ||||||||||||
| Transport | Room temperature in continental US; may vary elsewhere. | ||||||||||||
| Storage |
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| Reference | [1]. Kroflic A, et al. Thermodynamic characterization of 3-[(3-cholamidopropyl)-dimethylammonium]-1-propanesulfonate (CHAPS) micellization using isothermal titration calorimetry: temperature, salt, and pH dependence. Langmuir. 2012 Jul 17;28(28):10363-71. [2]. Menshikova I, et al. Nucleosomes structure and dynamics: effect of CHAPS. Int J Biochem Mol Biol. 2011;2(2):129-137. |