| Bioactivity | CFSE is a fluorescent dye that can penetrate the cell membrane. It can react with the free amine group in the cytoskeleton protein inside the cell, and finally form a protein complex with fluorescence. After entering the cell, CFSE locates in the cell membrane, cytoplasm and nucleus, and the fluorescence staining is strongest in the nucleus[1].CFSE dye can be uniformly inherited by the cells with cell division and proliferation, and its attenuation is proportional to the number of cell divisions. This phenomenon can be detected and analyzed by flow cytometry under the excitation light of 488nm, and can be used to detect the proliferation of cells[1]. |
| Invitro | CFSE 工作液的配制1.1 制备储存液用 DMSO 配制 10 mM 的 CFSE。如用 0.18 mL DMSO 溶解 1 mg CFSE。注：CFSE 储存液建议分装后于 -20 ℃ 或 -80 ℃ 避光保存。1.2 工作液的配制用预热好的无血清细胞培养基或 PBS 稀释储存液，配制成 5-10 μM 的 CFSE 工作液。注：请根据实际情况调整 CFSE 工作液浓度，且现用现配。细胞染色2.1 悬浮细胞：离心收集细胞，加入 PBS 洗涤两次，每次 5 分钟。贴壁细胞：弃去培养基，加入胰蛋白酶消化细胞。离心弃去上清后，加入 PBS 洗涤两次，每次 5 分钟。2.2 加入 1 mL CFSE 工作液，室温孵育 15 分钟。2.3 400 g，4℃ 离心 3-4 分钟，弃去上清。2.4 加入 PBS 洗涤细胞两次，每次 5 分钟。2.5 用 1 mL 无血清培养基或 PBS 重悬细胞后，在荧光显微镜或流式细胞仪下检测。 |
| Name | CFDA-SE |
| CAS | 150347-59-4 |
| Formula | C58H38N2O22 |
| Molar Mass | 557.46 |
| Appearance | Solid |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | -20°C, sealed storage, away from moisture and light *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light) |
| Reference | [1]. Banks HT, et al. Quantifying CFSE Label Decay in Flow Cytometry Data. Appl Math Lett. 2013 May 1;26(5):571-577. [2]. A Bruce Lyons, et al. Flow cytometric analysis of cell division by dilution of CFSE and related dyes. Curr Protoc Cytom. 2013;Chapter 9:Unit9.11. [3]. Cui J, et al. Prostaglandin E3 attenuates macrophage-associated inflammation and prostate tumour growth by modulating polarization. J Cell Mol Med. 2021 Jun;25(12):5586-5601. [4]. Xiuyun Jiang et al. Long-lived pancreatic ductal adenocarcinoma slice cultures enable precise study of the immune microenvironment. Oncoimmunology. 2017; 6(7): e1333210. |