Bioactivity | Blue Tetrazolium (Tetrazolium blue), as a blue dye for microbial research, can be reduced into blue tetrazolium formazan (BTF). Blue Tetrazolium can be used to determine the activity of succinate dehydrogenase (SDH) in yeast strains, which has been reduced as a substrate. Use DMSO to extract BTF from cells, and test absorption spectrum of BTF. The BTF shows a wide wavelength range of 480-600 nm with maximal absorbance seen at 540 nm. Blue Tetrazolium, combines with succinate dehydrogenase (SDH)[1]. |
Name | Blue Tetrazolium |
CAS | 1871-22-3 |
Formula | C40H32Cl2N8O2 |
Molar Mass | 727.64 |
Transport | Room temperature in continental US; may vary elsewhere. |
Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
Reference | [1]. Kregiel D, et al. Succinate dehydrogenase activity assay in situ with blue tetrazolium salt in crabtree-positive Saccharomyces cerevisiae strain[J]. Food Technology and Biotechnology, 2008, 46(4): 376-380. |