| Bioactivity | BZiPAR is a fluorescent probe. BZiPAR also is a substrate of trypsin that becomes fluorescent after cleavage by the protease[1]. |
| Invitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Confocal Imaging of Trypsinogen Activation:1. Incubate the cells according to your normal protocol.2. Isolate clusters of acinar cells.3. Incubate acini in extracellular solution containing 100 μM BZiPAR.4. BZiPAR were excited with a 488-nm laser line; emission was collected in the 508- to 530-nm band for cleaved BZiPAR. |
| Name | BZiPAR |
| CAS | 254451-46-2 |
| Formula | C70H88Cl2N14O13 |
| Molar Mass | 1404.44 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Mark W Sherwood, et al. Activation of trypsinogen in large endocytic vacuoles of pancreatic acinar cells. Proc Natl Acad Sci U S A. 2007 Mar 27;104(13):5674-9. |
BZiPAR
CAS: 254451-46-2 F: C70H88Cl2N14O13 W: 1404.44
BZiPAR is a fluorescent probe. BZiPAR also is a substrate of trypsin that becomes fluorescent after cleavage by the prot
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