| Bioactivity | BMPO (BocMPO) is a cell-permeable superior spin trap with favorable chemical and spectroscopic features. BMPO (BocMPO) can be used for detecting thiyl radicals, hydroxyl radicals, superoxide anions and glutathiyl radicals[1]. |
| Invitro | BMPO is able to trap both •OH and GS• to form more persistent BMPO/•OH and BMPO/•SG adducts. In addition, the electron spin resonance (ESR) spectra of BMPO-derived adducts exhibit high signal-to-noise ratios in biological systems. For example, the ESR spectrum of the BMPO glutathionyl adduct (BMPO/•SG) does not fully overlap with the spectrum of its hydroxyl adduct. The favorable chemical and spectroscopic features make BMPO ideal for the detection of superoxide anions, hydroxyl and thiyl radicals.[1]The potential toxic effects of the spin trap BMPO is measured by two estimates of cell viability (trypan blue exclusion and colony formation).[3]BMPO has no significant effect on cell viability at 2.5 or 25 mM in CHO cells. However, it leads to a significant increase in the number of cells that are unable to exclude Trypan blue at 50 mM BMPO. BMPO significantly reduces a 30% reduction in colony formation at 25 mM concentration in CHO cells. And, BMPO completely inhibits the colony formation of 9L tumor cells at 50 mM.[3]The cell function (rate of oxygen consumption) is also measured, BMPO (25 mM) significantly reduces the oxygen consumption rates in CHO cells.[3] |
| Name | BMPO |
| CAS | 387334-31-8 |
| Formula | C10H17NO3 |
| Molar Mass | 199.25 |
| Appearance | Solid |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | 4°C, sealed storage, away from moisture and light *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light) |
| Reference | [1]. H Zhao, et al. Synthesis and biochemical applications of a solid cyclic nitrone spin trap: a relatively superior trap for detecting superoxide anions and glutathiyl radicals. Free Radic Biol Med [2]. Hongtao Zhao, et al. Detection and characterization of the product of hydroethidine and intracellular superoxide by HPLC and limitations of fluorescence. Proc Natl Acad Sci U S A [3]. Nadeem Khan, et al. Spin traps: in vitro toxicity and stability of radical adducts. Free Radic Biol Med. 2003 Jun 1;34(11):1473-81. |