Ac-VDVAD-AFC_product_DataBase

Bioactivity	Ac-VDVAD-AFC is a caspase-specific fluorescent substrate. Ac-VDVAD-AFC can measure caspase-3-like activity and caspase-2 activity and can be used for the research of tumor and cancer[1].
Invitro	Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Labeling of Cells[1]:1. Culture cells in in a 6 well plate at a density of 3×105 cells per well or in a 96 well plate at a density of 3×104 cells per well. Incubate the cells according to your normal protocol. 2. 2 μM arsenic or cotreated with 2 μM arsenic and 25 μM quercetin to treate A549 cells for 24 h.3. 50 μL of cell lysate is made up to 200 μL buffer containing HEPES 20 mM, 40 μM DEVD‐AMC, or Ac‐VDVAD‐AFC, and 2mM dithiothreitol.4. 40 μM fluorescent substrate Ac-VDVAD-AFC is used to measure Caspase-2 activity for 4 days.5. Fluorescence is measured at 460 and 505 nm wavelengths, respectively (The activity was calculated as arbitrary fluorescence units per min per mg protein).
Name	Ac-VDVAD-AFC
CAS	210344-94-8
Formula	C33H41F3N6O12
Molar Mass	770.71
Transport	Room temperature in continental US; may vary elsewhere.
Storage	Please store the product under the recommended conditions in the Certificate of Analysis.
Reference	[1]. Pan Yang, et al. Quercetin attenuates the proliferation of arsenic-related lung cancer cells via a caspase-dependent DNA damage signaling. Mol Carcinog. 2022 Jul;61(7):655-663.

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