| Bioactivity | 8pyDTZ is a pyridyl diphenylterazine (DTZ) analog and an ATP-independent pyridyl substrate of LumiLuc luciferase. 8pyDTZ exhibits spectrally shifted emission. 8pyDTZ has excellent biocompatibility and superior in vivo sensitivity. 8pyDTZ can be used for in vivo luminescence imaging[1]. | ||||||
| Invitro | Compared to DTZ, 8pyDTZ (compounds 3c) is able to emit red-shifted chemiluminescence or bioluminescence. 8pyDTZ shows the most red-shifted emission and red-shifted photons can penetrate through tissue better[1].LumiLuc-8pyDTZ in human embryonic kidney (HEK) 293T cells transiently expressing the luciferase is evaluated. The LumiLuc-8pyDTZ pair produces ∼3-5-fold more bioluminescence than teLuc-8pyDTZ. To compare far-red emission intensities of bioluminescent reporters, HEK 293T cells in the presence of a 600-700 nm band pass filter are imaged. At substrate concentrations from 6.25 to 100 μM, LumiLuc-8pyDTZ consistently produces 1.6-3.9-fold higher photon flux than teLuc-DTZ. No ATP perturbation is observed from 8pyDTZ-treated, LumiLucexpressing cells[1]. | ||||||
| In Vivo | LumiLuc-8pyDTZ pair is compared with several benchmark reporters in a tumor xenograft mouse model. LumiLuc-8pyDTZ shows high sensitivity and does not need organic cosolvents for in vivo administration. LumiScarlet-8pyDTZ is comparable to Akaluc-AkaLumine, the brightest ATP-dependent luciferase-luciferin pair, for detecting cells in deep tissues of mice[1]. | ||||||
| Name | 8pyDTZ | ||||||
| CAS | 2351898-91-2 | ||||||
| Formula | C24H18N4O | ||||||
| Molar Mass | 378.43 | ||||||
| Appearance | Solid | ||||||
| Transport | Room temperature in continental US; may vary elsewhere. | ||||||
| Storage |
*该产品在溶液状态不稳定，建议您现用现配，即刻使用。 |
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| Reference | [1]. Yeh HW, et al. ATP-Independent Bioluminescent Reporter Variants To Improve in Vivo Imaging. ACS Chem Biol. 2019 May 17;14(5):959-965. |