| Bioactivity | 6-NBDG is a fluorescent glucose analogue, it can be used for fluorescence imaging and monitoring glucose transport and uptake. 6-NBDG can be used as a fluorescent probe for detecting macrophage-rich atherosclerotic plaques[1][2]. Storage: protect from light. |
| Invitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).1. Incubate sample with RPMI (without glucose) containing a final concentration of 200 μM 6-NBDG.2. Use a curved forcep to embeds sample into the mixture, and stretches the samplet through a drop of agarose (40°C, 20 μL) places on a pre-heated (40°C) glass slide.3. Gently place another preheated glass slide on the top of sample, so that the whole sample embeds into the agarose-6-NBDG mixture.4. Take confocal images with a confocal laser scanning microscope (Ex: 471 nm, Em: 500–600 nm)[2]. |
| Name | 6-NBDG |
| CAS | 108708-22-1 |
| Formula | C12H14N4O8 |
| Molar Mass | 342.26 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Hansen TV, et al. Glucose Absorption by the Bacillary Band of Trichuris muris. PLoS Negl Trop Dis. 2016 Sep 2;10(9):e0004971. [2]. Zaman RT, et al. Fiber-optic system for dual-modality imaging of glucose probes 18F-FDG and 6-NBDG in atherosclerotic plaques. PLoS One. 2014 Sep 18;9(9):e108108. |