| Bioactivity | 5-IAF (5-Iodoacetamidofluorescein) is an idoacetamide derivate of fluoresceine. 5-IAF can be used as fluorescent probe that labels proteins and other molecules having free thiols (cysteine side chains)[1][2]. | |||||||||
| Invitro | 8 µM GSH sample solution is derivatized with 8, 80, 200, 400, 800, 1600, 2000, 2500 µM 5-IAF, respectively and analyzed with CE-LIF. The highest signal intensity is obtained with 400 and 800 µM 5-IAF[2]. | |||||||||
| Name | 5-IAF | |||||||||
| CAS | 63368-54-7 | |||||||||
| Formula | C22H14INO6 | |||||||||
| Molar Mass | 515.25 | |||||||||
| Appearance | Solid | |||||||||
| Transport | Room temperature in continental US; may vary elsewhere. | |||||||||
| Storage |
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| Reference | [1]. Salvatore Sotgia, et al. Plasma L-Ergothioneine Measurement by High-Performance Liquid Chromatography and Capillary Electrophoresis after a Pre-Column Derivatization with 5-Iodoacetamidofluorescein (5-IAF) and Fluorescence Detection. PLoS One. 2013; 8(7): e70374. [2]. Yan Wang, et al. Determination of free and protein-bound glutathione in HepG2 cells using capillary electrophoresis with laser-induced fluorescence detection. J Chromatogr A. 2009 Apr 17;1216(16):3533-7. |