5-Ethynyluridine_product_DataBase

Bioactivity

5-Ethynyluridine (5-EU) is a potent cell-permeable nucleoside can be used to label newly synthesized RNA. 5-Ethynyluridine can be used for isolation and sequencing of nascent RNA from neuronal populations in vivo. 5-Ethynyluridine can be used to identify changes in transcription in vivo in nervous system disease models[1][2].

In Vivo

5-Ethynyluridine (75 mM in PBS for 1 µl; intracerebellar injection ) shows a labeling of central nervous system cells in mouse[1].5-Ethynyluridine (330-750 µMol/g; i.p.) results in in labelling of nascent RNA in cells of multiple organs and tissues, but not in the central nervous system in mouse[1].

Name

5-Ethynyluridine

CAS

69075-42-9

Formula

C11H12N2O6

Molar Mass

268.22

Appearance

Solid

Transport

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Reference

[1]. Van't Sant LJ, et al. In vivo 5-ethynyluridine (EU) labelling detects reduced transcription in Purkinje cell degeneration mouse mutants, but can itself induce neurodegeneration. Acta Neuropathol Commun. 2021 May 21;9(1):94. [2]. Jao CY, et al. Exploring RNA transcription and turnover in vivo by using click chemistry. Proc Natl Acad Sci U S A. 2008 Oct 14;105(41):15779-84.

PeptideDB

5-Ethynyluridine

CAS: 69075-42-9 F: C11H12N2O6 W: 268.22