Bioactivity | 4-Methylumbelliferyl β-D-Glucopyranoside, a β-D-glucoside, is a fluorogenic substrate for β-glucosidase, utilizes to assay β-glucosidase activity[1]. 4-Methylumbelliferyl β-D-Glucopyranoside releases the highly fluorescent 4-methylumbelliferyl (4-MU), which has an emission maximum at 445-454 nm. The excitation maximum for 4-MU is pH-dependent: 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively[2]. | ||||||||||||
Target | 4-Methylumbelliferyl β-D-Glucopyranoside is a fluorogenic substrate for β-glucosidase. | ||||||||||||
Name | 4-Methylumbelliferyl β-D-Glucopyranoside | ||||||||||||
CAS | 18997-57-4 | ||||||||||||
Formula | C16H18O8 | ||||||||||||
Molar Mass | 338.31 | ||||||||||||
Appearance | Solid | ||||||||||||
Transport | Room temperature in continental US; may vary elsewhere. | ||||||||||||
Storage |
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Reference | [1]. Smitka CM,et al. Rapid fluorogenic assay for differentiation of the Candida parapsilosis group from other Candida spp. J Clin Microbiol. 1989;27(1):203-206. [2]. Oftedal L, et al. Validation and assessment of preanalytical factors of a fluorometric in vitro assay for glucocerebrosidase activity in human cerebrospinal fluid. Sci Rep. 2020;10(1):22098. Published 2020 Dec 16. |