| Bioactivity | 4-Methylumbelliferyl phosphate (4-MUP) disodium, an anionic organophosphate, is a acid and alkaline phosphatase fluorogenic substrate. 4-Methylumbelliferyl phosphate disodium is also a nerve agent simulant[1][2][3]. |
| Invitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Serum acid phosphatase assay[1]:1. Preparation a 100 μL solution containing the following agents: 5.0 μL of serum enzyme, 50 μL of 4-Methylumbelliferyl phosphate (5.0 mM), and 10 μL of 1.0 M buffer at pH 6.0.2. Add the following agents (with the final concentration) 20 mM Sodium Tartrate (HY-128476), 15 mM Sodium Fluoride (HY-B1766), 3000 mM 2-Mercaptoethanol, 4 mM 1, 10-Phenanthroline (HY-W004544), 6 mM 8-Quinolinol (HY-B1005), 6 mM 8-Hydroxy-5quinolinesulfonic acid, 45 mM EDTA (HY-Y0682) and 6 mM EGTA (HY-D0861).3. Incubate reaction mixtures for 15 min at 37℃ and terminated by the addition of 2.9 mL of 0.1 M ammonium hydroxide/glycine buffer, pH 10.5.4. Mix samples thoroughly and determine fluorescence by a fluorometer. |
| Name | 4-Methylumbelliferyl phosphate disodium |
| CAS | 22919-26-2 |
| Formula | C10H7Na2O6P |
| Molar Mass | 300.11 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Chambers JP, et al. Determination of serum acid phosphatase in Gaucher's disease using 4-methylumbelliferyl phosphate. Clin Chim Acta. 1977 Oct 1;80(1):67-77. [2]. Watanabe F, et al. The analysis of alkaline phosphatase isoenzyme using 4-methylumbelliferyl phosphate as substrate on a cellulose acetate membrane. Clin Chim Acta. 1979 Feb 1;91(3):273-6. [3]. Hao Chen, et al. Autonomic Molecular Transport for Ultrasensitive Surface-Enhanced Infrared Absorption Spectroscopy. ACS Appl. Polym. Mater. 2020, 2, 9, 3929-3935 |
4-Methylumbelliferyl phosphate disodium
CAS: 22919-26-2 F: C10H7Na2O6P W: 300.11
4-Methylumbelliferyl phosphate (4-MUP) disodium, an anionic organophosphate, is a acid and alkaline phosphatase fluoroge
Sales Email：peptidedb@qq.com
This product is for research use only, not for human use. We do not sell to patients.