| CAS | 191529-67-6 |
| Sequence | H-Glu-Ala-Gly-Asp-Asp-Ile-Val-Pro-Cys-Ser-Met-Ser-Tyr-Thr-Trp-Thr-Gly-Ala-OH |
| Sequence Single | EAGDDIVPCSMSYTWTGA |
| Molecular Formula | C81H119N19O30S2 |
| Molecular Weight | 1903.08 |
| Synonyms | S5A/5B |
| Technology | Synthetic |
| Storage | -20°C, avoid light, cool and dry place |
| Application | Virology |
| Description | H-Glu-Ala-Gly-Asp-Asp-Ile-Val-Pro-Cys-Ser-Met-Ser-Tyr-Thr-Trp-Thr-Gly-Ala-OH also called S5A/5B, EAGDDIVPCSMSYTWTGA, which mimics the NS5A-5B cleavage junction of the nonstructural protein (NS) is an efficient substrate used by Shimuzu et al. to detect NS3 protease activity in a standard assay (Km = 411 µM and kcat = 0.14 min-1, without NS4 activator). Cleavage occurs between Cys and Ser. The cleavage products are analyzed by RP-HPLC. |