| CAS | 98849-88-8 |
| Sequence | H-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-OH |
| Sequence Single | DYKDDDDK |
| Molecular Formula | C41H60N10O20 |
| Molecular Weight | 1012.98 |
| Synonyms | Flag Peptide |
| Technology | Synthetic |
| Storage | -20°C, avoid light, cool and dry place |
| Description | FLAG Epitope Peptide also called Flag Peptide, consists of eight amino acids (DYKDDDDK) and represents a versatile fusion tag for the purification of recombinant proteins. It can be enzymatically removed with enterokinase which cleaves C-terminally to the tag sequence. FLAG Epitope Peptide is commonly used for the elution of immunoaffinity chromatography-purified recombinant proteins. |
| References | 1. Caspase inhibitors induce a switch from apoptotic to proinflammatory signaling in CD95-stimulated T lymphocytes. C.Scheller et al., Eur. J. Immunol., 32, 2471 (2002) 2. Topology of the Shaker potassium channel probed with hydrophilic epitope insertions. T.M.Shih and A.L.Goldin, J. Cell Biol., 136, 1037 (1997) 3. Minimal FLAG sequence useful in the functional epitope tagging of H-Ras. K.Y.Johnson et al., Biotechniques, 32, 1270 (2002) 4. A Short Polypeptide Marker Sequence Useful for Recombinant Protein Identification and Purification. A Short Polypeptide Marker Sequence Useful for Recombinant Protein Identification and Purification T.P.Hopp et al., Nat. Biotechnol., 6, 1204 (1988) |