Anthranilyl-HIV Protease Substrate HIV Peptides and HIV Protease Catalog

CAS	133233-38-2
Sequence	Abz-Thr-Ile-Nle-p-nitro-Phe-Gln-Arg-NH2
Sequence Single	Abz-TI-Nle-p-nitro-FQR-NH2
Molecular Formula	C43H65N13O11
Molecular Weight	940.07
Technology	Synthetic
Storage	-20°C, avoid light, cool and dry place
Application	Infectious Disease
Description	Anthranilyl-HIV Protease Substrate is a hexapeptide FRET substrate with the sequence of Abz-Thr-Ile-Nle-p-nitro-Phe-Gln-Arg-NH2, it is derived from the p24/p15 cleavage site of the viral gag-pol poly-protein. A simple, continuous fluorometric assay for HIV protease has been developed, which allows the screening of potential HIV protease inhibitors. Values for Km (2.1 μM) and kcat (7.4 s-1) were calculated from steady-state data at a low concentration of the enzyme. Excitation at 280 nm, emission at > 435 nm.
References	1. A simple, continuous fluorometric assay for HIV protease. M.W.Pennington et al., Peptides 1992, Proceedings of the 22nd European Peptide Symposium, Interlaken, Switzerland, p. 936, C.H.Schneider and A.N.Eberle, eds., Escom, Leiden, (1993)

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